Multiplex sequencing by hybridization.
Identifieur interne : 003432 ( Main/Exploration ); précédent : 003431; suivant : 003433Multiplex sequencing by hybridization.
Auteurs : E. Hubbell [États-Unis]Source :
- Journal of computational biology : a journal of computational molecular cell biology [ 1066-5277 ] ; 2001.
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Abstract
One of the limitations of classical sequencing by hybridization (SBH) is the inefficient use of probes in the "all k-mers" array. This limitation occurs due to the relatively short length (roughly the square root of C) of target that may be reconstructed by an array with C probes. We propose a new strategy, multiplex sequencing by hybridization, that greatly increases the efficiency of target reconstruction. In the typical multiplex SBH method, many different target sequences are simultaneously reconstructed (as compared to a single sequence in classic SBH). This is accomplished by pooling the target sequences and performing several hybridization experiments. This procedure makes more efficient use of probes so that the combined length of sequence reconstructed per DNA array increases significantly as compared to classical SBH.
DOI: 10.1089/106652701300312913
PubMed: 11454302
Affiliations:
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Le document en format XML
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Hubbell</name><affiliation wicri:level="4"><nlm:affiliation>Department of Mathematics, University of Southern California, Los Angeles, CA 90089-1113, USA. hubbell@hto.usc.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Mathematics, University of Southern California, Los Angeles, CA 90089-1113</wicri:regionArea><placeName><region type="state">Californie</region><settlement type="city">Los Angeles</settlement></placeName><orgName type="university">Université de Californie du Sud</orgName></affiliation></author></analytic><series><title level="j">Journal of computational biology : a journal of computational molecular cell biology</title><idno type="ISSN">1066-5277</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algorithms</term><term>Computer Simulation</term><term>Electronic Data Processing</term><term>In Situ Hybridization (methods)</term><term>Oligonucleotide Array Sequence Analysis (methods)</term><term>Sequence Analysis, DNA (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algorithmes</term><term>Analyse de séquence d'ADN ()</term><term>Hybridation in situ ()</term><term>Simulation numérique</term><term>Séquençage par oligonucléotides en batterie ()</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>In Situ Hybridization</term><term>Oligonucleotide Array Sequence Analysis</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Algorithms</term><term>Computer Simulation</term><term>Electronic Data Processing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algorithmes</term><term>Analyse de séquence d'ADN</term><term>Hybridation in situ</term><term>Simulation numérique</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">One of the limitations of classical sequencing by hybridization (SBH) is the inefficient use of probes in the "all k-mers" array. This limitation occurs due to the relatively short length (roughly the square root of C) of target that may be reconstructed by an array with C probes. We propose a new strategy, multiplex sequencing by hybridization, that greatly increases the efficiency of target reconstruction. In the typical multiplex SBH method, many different target sequences are simultaneously reconstructed (as compared to a single sequence in classic SBH). This is accomplished by pooling the target sequences and performing several hybridization experiments. This procedure makes more efficient use of probes so that the combined length of sequence reconstructed per DNA array increases significantly as compared to classical SBH.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Los Angeles</li></settlement><orgName><li>Université de Californie du Sud</li></orgName></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Hubbell, E" sort="Hubbell, E" uniqKey="Hubbell E" first="E" last="Hubbell">E. Hubbell</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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